Comparison of the antigenic relationship between Japanese encephalitis virus genotypes 1 and 3.
Kang BK, Hwang JM, Moon H, Han SY, Kim JM, Yang DK, Park BK, Song D.
Research Unit, Green Cross Veterinary Products, Yongin, Korea.
Animal and Plant Quarantine Agency, Anyang, Korea.
Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
College of Pharmacy, Korea University, Sejong, Korea. sds1@korea.ac.kr
Abstract
PURPOSE: The Japanese encephalitis virus (JEV) genotype circulating in Korea has changed from G3 to G1. Therefore, the purpose of this study was to compare the antigenic relationship between the two genotypes by using antibody tests. MATERIALS AND METHODS: Blood samples from 42 sows and 216 horses were collected, and their seroprevalence was monitored using the hemagglutination inhibition and virus neutralization tests. Antisera against JEV G1 and G3 were isolated and prepared from guinea pigs. The cross-reactivity of these two viruses was then compared using the neutralizing antibody test. RESULTS: We found that there was a difference in the seropositive ratios of JEV G1 and G3. However, the difference was dependent on the antibody test used. There was also an observed difference in the antigenicity between the two genotypes, as ascertained using the neutralizing antibody test. CONCLUSION: There is an evident difference in JEV antigenicity between the genotypes G1 and G3. Therefore, we propose monitoring of the seroprevalence of JEV, and reevaluating the antigenicity of the current vaccine by using the relevant tests.
Clin Exp Vaccine Res. 2016 Jan;5(1):26-30. https://doi.org/10.7774/cevr.2016.5.1.26