동식물위생연구 국제공인연구실 WOAH reference lab 브루셀라병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 77차 총회에서 아·태 지역 최초로 브루셀라병 표준실험실로 인정받음(2009년 5월)
이진주 수의연구사
[농림축산검역본부]
실험실-세균질병과
[농림축산검역본부]
Immunogenic proteins of Brucella abortus to minimize cross reactions in Brucellosis diagnosis(2012-01-10) | |||||
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담당부서 | Animal and Plant Health Research | 작성자 | Moon Her | 0 | 2012-01-10 |
Kyumg Yuk Ko, Jong Wan Kim, Moon Her, Sung-Il Kang, Suk-Chan Jung, Donghee Cho, Ji-Yeon Kim*. 2011. Immunogenic proteins of Brucella abortus to minimize cross reactions in Brucellosis diagnosis. Vet. Microbiol. Doi:10.1016/j.vetmic.2011.11.011. AbstractTo overcome the limitations of serological diagnosis, including false positive reactions caused by other pathogens, specific antigens for diagnosis of brucellosis other than LPS have been required. The present study was conducted to separate and identify immuno-dominant insoluble proteins of Brucella abortus against the antisera of cattle infected with B. abortus, or/and Yersinia enterocolitica, or the sera of non-infected cattle. After separating insoluble proteins of B. abortus by two dimensional electrophoresis (2-DE), their immuno-reactivity was determined by western blotting. A portion of the immunogenic spots against the positive antisera of B. abortus that have the potential for use as specific antigens were identified by MS/MS analysis. Overall, 18 immunogenic insoluble proteins of B. abortus 1119-3 showed immuno-reactivity against only the positive antisera of B. abortus, but failed to have immunogenicity toward both the positive sera of Y. enterocolitica and the negative sera of B. abortus. Identification of these proteins revealed the following: F0F1 ATP synthase subunit β, solute-binding family 5 protein, 28kDa OMP, Leu/Ile/Val-binding family protein, Histidinol dehyddrogenase, Hypothetical protein, Twin-arginine translocation pathway signal sequence domain-containing protein, Dihydroorotase, Serine protease family protein, β-hydroxyacyl-(acyl-carrier-protein) dehydratase FabA, Short-chain dehydrogenase-/reductase carbonic anhydrase, Orinithine carbamoyltransferase, Leucyl aminopeptidase, Cold shock DNA-binding domain-containing protein, Cu/Zn superoxide dismutase, and Methionine aminopeptidase. The 18 immunogenic proteins separated in the present study can be considered candidate antigens to minimize cross reaction in the diagnosis of brucellosis and useful sources for Brucella vaccine development. |
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