동식물위생연구 국제공인연구실 WOAH reference lab 광견병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 80차 총회에서 광견병 표준실험실로 인정받음
(2012년 5월)
양동군 수의연구관
[농림축산검역본부]
실험실-바이러스질병과
[농림축산검역본부]
Establishment of multiplex RT-PCR for differentiation between rabies virus with and that without mutation at position 333 of glycoprotein(2021-04-02) | |||||
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담당부서 | 동식물위생연구부 | 작성자 | 유재영 | 0 | 2021-04-02 |
ABSTRACT Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use as a bait vaccine. Therefore, new means of differentiating ERAGS from other rabies virus (RABV) strains will be required in biological manufacturing and diagnostic service centers. In this study, we designed two specific primer sets for differentiation between ERAGS and other RABVs based on mutation in the RABV glycoprotein gene. Polymerase chain reaction analysis of the glycoprotein gene revealed two DNA bands of 383 bp and 583 bp in the ERAGS strain but a single DNA band of 383 bp in the field strains. The detection limits of multiplex reverse transcription polymerase chain reaction (RT-PCR) were 80 and 8 FAID50/reaction for the ERAGS and Evelyn-Rokitnicki-Abelseth strains, respectively. No cross-reactions were detected in the non-RABV reference viruses, including canine distemper virus, parvovirus, canine adenovirus type 1 and 2, and parainfluenza virus. The results of multiplex RT-PCR were 100% consistent with those of the fluorescent antibody test. Therefore, one-step multiplex RT-PCR is likely useful for differentiation between RABVs with and those without mutation at position 333 of the RABV glycoprotein gene. J Vet Sci. 2020 Mar;21(2):e22 |
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첨부파일 | 1개 / 1745KB (전체저장 버튼 클릭 시 압축파일로 저장됩니다.)
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