동식물위생연구 국제공인연구실 WOAH reference lab 브루셀라병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 77차 총회에서 아·태 지역 최초로 브루셀라병 표준실험실로 인정받음(2009년 5월)
이진주 수의연구사
[농림축산검역본부]
실험실-세균질병과
[농림축산검역본부]
Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis(2013-05-16) | |||||
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담당부서 | 동식물위생연구부 | 작성자 | Moon Her | 0 | 2013-05-16 |
So Ra Sung, Ji Yeon Kim, Moon Her, Ki Chan Lee, Jeong Hui Gu, Sung Il Kang, Hyang Keun Lee, Suk Mi Kim, Suk Chan Jung Korean Journal of Veterinary Service 2012, 35(4), 269-273 Abstract A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diag-nostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional vet-erinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked im-munosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three pre-scribed tests ranged high 84.1∼97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea. |
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