On going study for the production of monoclonal antibodies for aflatoxin B1 and zearalenoe ( 08/07/31 ) | |||||
---|---|---|---|---|---|
Part | Charger | vetzang | date | 08/07/31 | |
Bio-application lab. in Veterinary Biotechnology Division has prepared aflatoxin B1conjugate and zearalenone conjugate to produce monoclonal antibodies for each mycotoxin.
Parent toxin was firstly reacted with carboxymethoxylamine(CMO) to give functional group (NOCH2COOH) in aflatoxin (1-position) and zearalenone.
After purification and confirmation, haptenized aflatoxin B1 and zearalenone were further conjugated with bovine serum albumin. Conjugated mycotoxin were purified or salted with Sephadex 25 column or PD10 centrifuge filter.
Conjugated aflatoxin B1 or zearalenone will have been adminitered to female Balb/C mouse via IP till end of September.
|
|||||
file | 0개
(전체저장 버튼 클릭 시 압축파일로 저장됩니다.)
|
||||