동식물위생연구 국제공인연구실 WOAH reference lab 브루셀라병연구실
동식물위생연구에 대한 모든 정보가 있습니다.
WOAH 77차 총회에서 아·태 지역 최초로 브루셀라병 표준실험실로 인정받음(2009년 5월)
이진주 수의연구사
[농림축산검역본부]
실험실-세균질병과
[농림축산검역본부]
Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis.(2015-04-14) | |||||
---|---|---|---|---|---|
담당부서 | 동식물위생연구부 | 작성자 | 이진주 | 0 | 2015-04-14 |
Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis. Comp Immunol Microbiol Infect Dis. 2014 Sep;37(4):221-8. Lee JJ, Lim JJ, Kim DG, Simborio HL, Kim DH, Reyes AW, Min W, Lee HJ, Kim DH, Chang HH, Kim S. Abstract In this study, we characterized the secreted proteins of Brucella abortus into the enriched media under the bacterial laboratory growth condition and investigated the pathogenic importance of culture supernatant (CS) proteins to B. abortus infection. CS proteins from stationary phase were concentrated and analyzed using 2D electrophoresis. In MALDI TOF/TOF analysis, more than 27 proteins including CuZn SOD, Dps, Tat, OMPs, Adh, LivF, Tuf, SucC, GroEL and DnaK were identified. Cytotoxic effects of CS proteins were found to increase in a dose-dependent manner in RAW 264.7 cells. Upon B. abortus challenge into phagocytes, however, CS proteins pre-treated cells exhibited lower bacterial uptake and intracellular replication compared to untreated cells. Immunization with CS proteins induced a strong humoral and cell mediated immune responses and exhibited significant higher degree of protection against virulence of B. abortus infection compared to mice immunized with Brucella broth protein (BBP). Taken together, these results indicate that B. abortus secreted a number of soluble immunogenic proteins under laboratory culture condition, which can promote antibody production resulted in enhancing host defense against to subsequently bacterial infection. Moreover, further analysis of CS proteins may help to understand the pathogenic mechanism of B. abortus infection and host-pathogen interaction. |
|||||
첨부파일 | 0개 (전체저장 버튼 클릭 시 압축파일로 저장됩니다.)
|
||||
|